Strain Information
| Name | WBM1340 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | wbmIs99 IV. |
| Description | wbmIs99 [rab-3p::3xFLAG::rpl-22::SL2::wrmScarlet::rab-3 3'UTR, *wbmIs89] IV. N-terminal 3x flag tagged RPL-22 ribosomal subunit expressed in the nervous system. wrmScarlet expression in nervous system. Can be used for Single-copy Knock-In Translating Ribosome Immunoprecipitation (SKI TRIP) experiments. Derived by modification of wbmIs89. Reference: Wester LE, et al. Cell Rep. Methods 2023 3, 100433. 10.1016/j.crmeth.2023.100433 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x6 |
| Made by | Anne Lanjuin |
| Laboratory | WBM |
| Reference | Wester, L.E., Lanjuin, A., Bruckisch, E.H.W., Perez-Matos, M.C., Stine, P.G., Heintz, C., Denzel, M.S., and Mair, W.B. (2023) A single-copy knockin translating ribosome immunoprecipitation toolkit for tissue-specific profiling of actively translated mRNAs in C. elegans. Cell Rep. Methods 3, 100433. 10.1016/j.crmeth.2023.100433 |
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