Strain Information

Name WBM1339   View On Wormbase
Species C. elegans
GenotypewbmIs118 I.
DescriptionwbmIs118 [myo-3p::3XFLAG::rpl-22::SL2::wrmScarlet::unc-54 3'UTR, *wbmIs114] I. N-terminal 3x flag tagged RPL-22 ribosomal subunit expressed in muscle. wrmScarlet expression in muscle. Can be used for Single-copy Knock-In Translating Ribosome Immunoprecipitation (SKI TRIP) experiments. Derived by modification of wbmIs114. Reference: Wester LE, et al. Cell Rep. Methods 2023 3, 100433. 10.1016/j.crmeth.2023.100433
Made byAnne Lanjuin
Laboratory WBM
Reference Wester, L.E., Lanjuin, A., Bruckisch, E.H.W., Perez-Matos, M.C., Stine, P.G., Heintz, C., Denzel, M.S., and Mair, W.B. (2023) A single-copy knockin translating ribosome immunoprecipitation toolkit for tissue-specific profiling of actively translated mRNAs in C. elegans. Cell Rep. Methods 3, 100433. 10.1016/j.crmeth.2023.100433
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