Strain Information
| Name | WBM1250 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | raga-1(wbm40[raga-1::AID::EmGFP]) II; wbmIs83 IV. |
| Description | wbmIs83 [rab-3p::3xFlag::TIR1::rab-3 3'UTR] *wbmIs66 IV. Auxin-inducible degron (AID) and EmGFP tags inserted at the C-terminus of the endogenous raga-1 locus using CRISPR/Cas9. Neuronal-specific expression of TIR1 allows for auxin-inducible degradation of RAGA-1 in the nervous system. wbmIs66 [rab-3p::3XFLAG::dpy-10 crRNA::rab-3 3'UTR] (IV:5015000). Reference: Smith HJ, et al. PLOS Genetics 19(9): e1010938. https://doi.org/10.1371/journal.pgen.1010938. PMID: 37721956. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x6 |
| Made by | Caroline Heintz and Hannah J Smith |
| Laboratory | WBM |
| Reference | Smith HJ, Lanjuin A, Sharma A, Prabhakar A, Nowak E, et al. (2023) Neuronal mTORC1 inhibition promotes longevity without suppressing anabolic growth and reproduction in C. elegans. PLOS Genetics 19(9): e1010938. https://doi.org/10.1371/journal.pgen.1010938 |
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