Strain Information

Name WBM1153   View On Wormbase
Species C. elegans
GenotypewbmIs72 III.
DescriptionwbmIs72 [pie-1p::3XFLAG::GFP::unc-54 3'UTR *wbmIs60] (III:7007600). Superficially wild-type. SKI LODGE system allows for CRISPR knock-in of single-copy transcripts downstream of a tissue-specific promoter. wbmIs72 exhibits germline-specific GFP expression driven the pie-1 promoter. Derived from parental strain WBM1119 by CRISPR-mediated insertion of GFP downstream of tissue-specific pie-1 promoter inserted as a single copy into the C. elegans genome (wbmIs60). Reference: Silva-García CG, et al. G3 (Bethesda). 2019 Jul 9;9(7):2195-2198.
Made byCarlos G Silva-García
Laboratory WBM
Reference Carlos G Silva-García, Caroline Heintz, Sneha Dutta, Nicole M Clark, Anne Lanjuin, William B Mair. Single-copy Knock-In Loci for Defined Gene Expression in C. elegans. BioRxiv. December 19, 2018 doi:
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