Strain Information

Name WBM1143   View On Wormbase
Species C. elegans
GenotypewbmIs67 V.
DescriptionwbmIs67 [eft-3p::3XFLAG::wrmScarlet::unc-54 3'UTR *wbmIs65] (V:8645000). Superficially wild-type. SKI LODGE system allows for CRISPR knock-in of single-copy transcripts downstream of a tissue-specific promoter. wbmIs67 exhibits soma-specific wrmScarlet expression driven the eft-3 promoter. Derived from parental strain WBM1140 by CRISPR-mediated insertion of wrmScarlet downstream of tissue-specific eft-3 promoter inserted as a single copy into the C. elegans genome (wbmIs65). Reference: Silva-García CG, et al. G3 (Bethesda). 2019 Jul 9;9(7):2195-2198.
MutagenCrispr/Cas9
Outcrossedx6
Made byCarlos G Silva-García
Laboratory WBM
Reference Carlos G Silva-García, Caroline Heintz, Sneha Dutta, Nicole M Clark, Anne Lanjuin, William B Mair. Single-copy Knock-In Loci for Defined Gene Expression in C. elegans. BioRxiv. December 19, 2018 doi: https://doi.org/10.1101/500892
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