Strain Information

Name WBM1126   View On Wormbase
Species C. elegans
GenotypewbmIs61 I.
DescriptionwbmIs61 [myo-3p::3XFLAG::dpy-10 crRNA::unc-54 3'UTR] (I:2851000). Superficially wild-type. SKI LODGE system allows for CRISPR knock-in of single-copy transcripts downstream of a tissue-specific promoter. wbmIs61 can be used to direct muscle-specific gene expression from the myo-3 promoter through CRISPR-mediated insertion of transcripts downstream of tissue-specific myo-3 promoter inserted as a single copy into the C. elegans genome. Reference: Silva-García CG, et al. G3 (Bethesda). 2019 Jul 9;9(7):2195-2198.
MutagenCrispr/Cas9
Outcrossedx6
Made byCarlos G Silva-García
Laboratory WBM
Reference Carlos G Silva-García, Caroline Heintz, Sneha Dutta, Nicole M Clark, Anne Lanjuin, William B Mair. Single-copy Knock-In Loci for Defined Gene Expression in C. elegans. BioRxiv. December 19, 2018 doi: https://doi.org/10.1101/500892
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