Strain Information
| Name | WBM1119 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | wbmIs60 III. |
| Description | wbmIs60 [pie-1p::3XFLAG::dpy-10 crRNA::unc-54 3'UTR] (III:7007600). Superficially wild-type. SKI LODGE system allows for CRISPR knock-in of single-copy transcripts downstream of a tissue-specific promoter. wbmIs60 can be used to direct germline-specific gene expression from the pie-1 promoter through CRISPR-mediated insertion of transcripts downstream of tissue-specific pie-1 promoter inserted as a single copy into the C. elegans genome. Reference: Silva-García CG, et al. G3 (Bethesda). 2019 Jul 9;9(7):2195-2198. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x6 |
| Made by | Carlos G Silva-García |
| Laboratory | WBM |
| Reference | Carlos G Silva-García, Caroline Heintz, Sneha Dutta, Nicole M Clark, Anne Lanjuin, William B Mair. Single-copy Knock-In Loci for Defined Gene Expression in C. elegans. BioRxiv. December 19, 2018 doi: https://doi.org/10.1101/500892 |
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