Strain Information

Name SZ355   View On Wormbase
Species C. elegans
Genotypeunc-73(az63) dxbp-1(az52) I; smg-4(az152) V.
Descriptionaz52 is a CRISPR-engineered M107I missense allele of dxbp-1. az52 has no phenotype on its own, but suppresses unc-73(e936) and unc-73(az63) by promoting use of a cryptic splice site for an intron beginning with UU. smg-4(az152) provides an NMD deficient background allowing identification of out-of-frame mis-splicing in an RNA-seq experiment. Reference: Suzuki JMNGL, et al. PLoS Genet. 2022 Feb 10;18(2):e1010028. doi: 10.1371/journal.pgen.1010028. PMID: 35143478.
Made byAlan Zahler
Laboratory SZ
Reference Suzuki JMNGL, Osterhoudt K, Cartwright- Acar CH, Gomez DR, Katzman S, Zahler AM (2022) A genetic screen in C. elegans reveals roles for KIN17 and PRCC in maintaining 5’ splice site identity. PLoS Genet 18(2): e1010028. https://doi. org/10.1371/journal.pgen.1010028 See S1 text for complete sequence of all alleles
Sign in or register an account if you want to order this strain.