Strain Information
| Name | SZ355 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-73(az63) dxbp-1(az52) I; smg-4(az152) V. |
| Description | az52 is a CRISPR-engineered M107I missense allele of dxbp-1. az52 has no phenotype on its own, but suppresses unc-73(e936) and unc-73(az63) by promoting use of a cryptic splice site for an intron beginning with UU. smg-4(az152) provides an NMD deficient background allowing identification of out-of-frame mis-splicing in an RNA-seq experiment. Reference: Suzuki JMNGL, et al. PLoS Genet. 2022 Feb 10;18(2):e1010028. doi: 10.1371/journal.pgen.1010028. PMID: 35143478. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Alan Zahler |
| Laboratory | SZ |
| Reference | Suzuki JMNGL, Osterhoudt K, Cartwright- Acar CH, Gomez DR, Katzman S, Zahler AM (2022) A genetic screen in C. elegans reveals roles for KIN17 and PRCC in maintaining 5’ splice site identity. PLoS Genet 18(2): e1010028. https://doi. org/10.1371/journal.pgen.1010028 See S1 text for complete sequence of all alleles |
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