Strain Information

Name SZ345   View On Wormbase
Species C. elegans
Genotypeunc-73(e936az30) dxbp-1(az121) I; smg-4(az152) V.
Descriptiondxbp-1(az121) is a K23N mutation that promotes usage of introns starting in UU when the sequence GUU is present at the 5' end of the intron. az121 was initially identified as able to suppress uncoordination of unc-73(e936) by promoting a cryptic splice site that defines an intron beginning UU. smg-4 mutant background allows for high throughput sequencing to identify frame shifted transcripts since it can move the 5'ss over by 1nt. e936az30 has no phenotype on its own, but it offers two adjacent cryptic 5'ss separated by 1nt. Reference: Suzuki JMNGL, et al. PLoS Genet. 2022 Feb 10;18(2):e1010028. doi: 10.1371/journal.pgen.1010028. PMID: 35143478.
Made byAlan Zahler
Laboratory SZ
Reference Suzuki et al. (2022) A genetic screen in C. elegans reveals roles for KIN17 and PRCC in maintaining 5’ splice site identity. PLoS Genet 18(2): e1010028. https://doi. org/10.1371/journal.pgen.1010028 See S1 text from that manuscript for full allele sequence
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