Strain Information
| Name | SZ340 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | smg-4(az152) V. |
| Description | CRISPR/Cas9 engineered smg-4 null allele. smg-4(az152) allele is confirmed NMD-defective by both the presence of the protruding vulva phenotype and the accumulation of NMD-targeted isoforms. smg-4(az152) is easy to track in crosses by PCR and digestion with BstBI (see S1 text of Suzuki, et al. for sequence of allele) and essentially mimics ma116 in having a G->A mutation at the last base of intron 1. az152 also removes two bases of exon 2 and inserts 50nt in exon 2. Reference: Suzuki JMNGL, et al. PLoS Genet. 2022 Feb 10;18(2):e1010028. doi: 10.1371/journal.pgen.1010028. PMID: 35143478. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x1 |
| Made by | Alan Zahler |
| Laboratory | SZ |
| Reference | Suzuki et al. (2022) A genetic screen in C. elegans reveals roles for KIN17 and PRCC in maintaining 5’ splice site identity. PLoS Genet 18(2): e1010028. https://doi. org/10.1371/journal.pgen.1010028 |
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