Strain Information

Name SV1897   View On Wormbase
Species C. elegans
Genotypeunc-119(ed3) III; oxTi970 swsn-4(he277[K564A]) IV/swsn-4(he268 he272 [LoxN start + LoxN intron 5]) IV; heSi208 V; heSi141 X.
DescriptionoxTi970 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. heSi208 [eft- 3p::LoxP::NLS(egl-13)::tagBFP2::tbb-2 UTR::LoxP::NLS(egl-13)::mCherry::tbb-2 3'UTR] V. heSi141 [hlh-8p::CRE] X. Heterozygous. Pick GFP+ to maintain. he277 is a kinase-dead version of swsn-4 linked to oxTi970 maintained over a functional copy of swsn-4 carrying LoxN insertions. he277 homozygotes are larval lethal. he268 he272 homozygotes are non-GFP and Egl since they cannot form a functioning vulva due to swsn-4 inactivated in the mesoderm lineage by hlh-8p::CRE expression. LoxN sites in the endogenous swsn-4 locus facilitate inducible knockout of swsn-4. Reference: van der Vaart A, et al. Sci Adv 2020 May 20;6(21):eaay3823. PMID: 32494730
MutagenCrispr/Cas9
Outcrossedx0
Made byAniek van der Vaart
Laboratory SV
Reference A. van der Vaart, M. Godfrey, V. Portegijs, S. van den Heuvel, Dose-dependent functions of SWI/SNF BAF in permitting and inhibiting cell proliferation in vivo. Sci. Adv. 6, eaay3823 (2020).
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