Strain Information
| Name | SSM596 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rpa-1(iow117)/mIn1[mIs14 dpy-10(e128)] II. |
| Description | Crispr/Cas9-engineered indel in the 5’ region of rpa-1. Larval-lethal mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP iow117 homozygotes (larval lethal). Pick wild-type dim GFP and check for correct segregation of progeny to maintain. iow117 was generated in mre-11::GFP background and outcrossed to N2. Reference: Hefel et al., Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x1 |
| Made by | Adam Hefel |
| Laboratory | SSM |
| Reference | Hefel et al Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293. |
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