Strain Information

Name SSM474   View On Wormbase
Species C. elegans
Genotypesyp-4(iow90[GFP11::syp-4]) I; iowSi8 II; unc-119(ed3) III.
Descriptionsyp-4(iow90[GFP11::syp-4]) I. iowSi8[pie-1p::GFP(1-10)::him-3 3UTR + Cbr-unc119(+)] II. CRISPR/Cas9 insertion of GFP11 tag into endogenous syp-4 locus in background strain SSM471. GFP::SYP-4 fluorescence only observed in the germline. If germline silencing occurs, transgene expression can be recovered by growing worms at 25C for 2 generations. Reference: Hefel A & Smolikove S. G3. 2019 Jun 5;9(6):1933-1943. PMID: 30992318.
Mutagen
Outcrossedx0
Made byAdam Hefel
Laboratory SSM
Reference G3. 2019 Jun 5;9(6):1933-1943. “Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in the Caenorhabditis elegans Germline” PMID: 30992318.
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