Strain Information
| Name | SSM473 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rpa-1(iow89[GFP11::rpa-1]) II; iowSi8 II; unc-119(ed3) III. |
| Description | rpa-1(iow89[GFP11::rpa-1]) II. iowSi8 [pie-1p::GFP1-10::him-3 3’UTR + Cbr-unc119(+)] II. CRISPR/Cas9 insertion of GFP11 tag into endogenous rpa-1 locus in background strain SSM471. GFP::RPA-1 fluorescence only observed in the germline. If germline silencing occurs, transgene expression can be recovered by growing worms at 25C for 2 generations. Reference: Hefel A & Smolikove S. G3. 2019 Jun 5;9(6):1933-1943. PMID: 30992318. |
| Mutagen | MosSCI |
| Outcrossed | x0 |
| Made by | Adam Hefel |
| Laboratory | SSM |
| Reference | G3. 2019 Jun 5;9(6):1933-1943. “Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in the Caenorhabditis elegans Germline” PMID: 30992318. |
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