Strain Information

Name SSM472   View On Wormbase
Species C. eleagns
Genotypeakir-1(iow88[GFP11::akir-1]) I; iowSi8 II; unc-119(ed3) III.
Descriptionakir-1(iow88[GFP11::akir-1]) I. iowSi8[pie-1p::GFP(1-10)::him-3 3UTR + Cbr-unc119(+)] II. CRISPR/Cas9 insertion of GFP11 tag into endogenous akir-1 locus in background strain SSM471. GFP::AKIR-1 fluorescence only observed in the germline. If germline silencing occurs, transgene expression can be recovered by growing worms at 25C for 2 generations. Reference: Hefel A & Smolikove S. G3. 2019 Jun 5;9(6):1933-1943. PMID: 30992318.
MutagenMosSCI
Outcrossedx0
Made byAdam Hefel
Laboratory SSM
Reference G3. 2019 Jun 5;9(6):1933-1943. “Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in the Caenorhabditis elegans Germline” PMID: 30992318.
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