Strain Information
| Name | SSM291 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ubc-9(iow31[3xFLAG::ubc-9]) IV/nT1[qIs51] (IV;V). |
| Description | Heterozygotes are wild-type with pharyngeal-expressed GFP, and segregate wild-type GFP+ heterozygotes, arrested nT1[qIs51] homozygotes, and viable non-GFP ubc-9(iow31[3xFLAG::ubc-9]) homozygotes. ubc-9(iow31[3xFLAG::ubc-9]) homozygotes produce 100% dead embryos, but cytologically they have normal germline (examined by DAPI and RAD-51 staining), suggesting that 3xFLAG::ubc-9 is functional in the gonad but not in the non-maternally rescued embryo. Maintain the strain by picking GFP+ worms and check for fertile non-GFP (which would indicate balancer has broken down). 3XFLAG added by CRISPR/Cas9. Synonymous point mutation where included in the repair template (positions 30, 33 and 36 of coding sequence). Reference: Reichman R, et al. Genetics. 2018 Apr;208(4):1421-1441. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Rachel Reichman |
| Laboratory | SSM |
| Reference | Reichman R, Shi Z, Malone R, Smolikove S. Genetics. 2018 Apr;208(4):1421-1441. |
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