Strain Information

Name RSL123   View On Wormbase
Species C. elegans
Genotypedpy-10(cn64) rab-3(ftw79) II.
Descriptionrab-3(ftw79[rab-3::SL2::LoxP::GFP::NLS::3'UTR::Abeta(inv)::s
igPep(inv)::T2A(inv)::wrmScarlet11(inv)::LoxP(inv)])
II. Modified endogenous rab-3 locus co-expresses stochastic, switchable cassette by trans-splicing using CRISPR-Cas9. Green fluorescence visible in neurons by dissection fluorescence microscopy. Inverted LoxP sites flank the GFP-NLS and inverted wrmScarlet11::T2A::signalPeptide::Abeta insert. Please contact Ryan Littlefield prior to publishing work using this strain.
MutagenCrispr/Cas9
Made byRyan Littlefield
Laboratory RSL
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