Strain Information
| Name | PD5998 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rpl-5(cc5998)/mIn1 [dpy-10(e128) mIs14] II. |
| Description | Balancer recombination happens frequently at 23-25C, strain must be maintained at 16-20C. Homozygous lethal mutation balanced by Dpy- and myo-2p::GFP-marked inversion. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP+, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP rpl-5(cc5998) homozygotes. Pick wild-type GFP+ to maintain. cc5998 is an engineered mutation creating an early stop (A166*). Presumptive rpl-5 null. Heterozygous rpl-5(cc5998)/mIn1 animals are delayed in development. Check for proper segregation of progeny. Reference: Cenik ES, et al. Dev Cell. 2019 Mar 25;48(6):811-826.e6. doi: 10.1016/j.devcel.2019.01.019. PMID: 30799226. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x1 |
| Made by | Elif Sarinay Cenik/ Agustian Surya |
| Laboratory | ESC |
| Reference | Cenik ES, Meng X, Tang NH ,Hall RN, Arribere JA, Cenik C, Jin Y, Fire AZ (2019). Maternal ribosomes are sufficient for tissue diversification during embryonic development in C. elegans. Developmental Cell, Mar25;48(6), 811-826.e6. , highlighted in the same issue with a preview by Haag & Dinman, 2019, Featured in Faculty of 1000. |
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