Strain Information
| Name | PD5994 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rps-23(cc5994)/tmC5 [F36H1.3(tmIs1220)] IV. |
| Description | Balancer recombination happens frequently at 23-25C, strain must be maintained at 16-20C. Homozygous lethal mutation balanced by myo-2p::Venus-marked inversion. Heterozygotes are wild-type with somewhat dimmer Venus signal and segregate WT Venus(+) heterozygotes, Mec Unc Venus(+) tmC5 homozygotes, and non-Venus rps-23(cc5994) homozygotes (L1 arrest). Pick wild-type Venus(+) and check for proper segregation of progeny to maintain. cc5994 is an engineered mutation creating an early stop (A67*). Presumptive rps-23 null. Heterozygous rps-23(cc5994)/tmC5 animals are delayed in development. Check for proper segregation of progeny. Reference: Cenik ES, et al. Dev Cell. 2019 Mar 25;48(6):811-826.e6. doi: 10.1016/j.devcel.2019.01.019. PMID: 30799226. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | Elif Sarinay Cenik/Agustian Surya |
| Laboratory | ESC |
| Reference | Cenik ES, Meng X, Tang NH ,Hall RN, Arribere JA, Cenik C, Jin Y, Fire AZ (2019). Maternal ribosomes are sufficient for tissue diversification during embryonic development in C. elegans. Developmental Cell, Mar25;48(6), 811-826.e6. , highlighted in the same issue with a preview by Haag & Dinman, 2019, Featured in Faculty of 1000. |
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