Strain Information
| Name | OG1156 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ogt-1(dr93[delta-TPR domain]) III; drIs4 IV. |
| Description | drIs4 [gpdh-1p::GFP + col-12p::DsRed] IV. TPR domain deleted in the endogenous ogt-1 locus using CRISPR/Cas9; Sanger sequence confirmed. The TPR domain deletion (128 aa - 583 aa) ablates the O-GlcNAcylation activity of OGT-1 as measured by the RL2 O-GlcNAc antibody. Defective gpdh-1p::GFP induction in the hypodermis and intestine during hypertonic stress. Constitutive col-12p::DsRed expression. Impaired adaptation to hypertonic stress. Reference: Urso SJ, et al. (2020). The O-GlcNAc transferase OGT is a conserved and essential regulator of the cellular and organismal response to hypertonic stress. bioRxiv, 2020.2005.2001.072033. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Sarel Urso |
| Laboratory | OG |
| Reference | Urso, S.J., Comly, M., Hanover, J.A., and Lamitina, T. (2020). The O-GlcNAc transferase OGT is a conserved and essential regulator of the cellular and organismal response to hypertonic stress. bioRxiv, 2020.2005.2001.072033. |
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