Strain Information
| Name | NOB142 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | daf-2(e1370) III; isp-1(qm150) IV/nT1[qIs51] (IV;V). |
| Description | Maintain at 15-20C: will form dauers at higher temps. Slow growing. Pick GFP+ to maintain. Heterozygotes are GFP+ and segregate WT GFP+ hets, arrested nT1[qIs51] aneuploids, and non-GFP daf-2(e1370); isp-1(qm150) homozygotes (sterile). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. [NOTE: Originally published daf-2(e1370); isp-1(qm150) strain MQ876 was found to not actually contain the daf-2(e1370) mutation. This strain was made to study the double mutant and revealed that daf-2(e1370); isp-1(qm150) homozygotes are inviable/sterile.] Reference: Cooper et al. In preparation. |
| Mutagen | No mutagen |
| Outcrossed | x3 |
| Made by | Nick Burton |
| Laboratory | NOB |
| Reference | Preprint available (Cooper et al., 2024). Paper as yet unpublished. |
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