Strain Information

Name MSB115   View On Wormbase
Species C elegans
Genotypeunc-70(mir6[loxP] mir16[loxP]) V.
DescriptionSuperficially wild-type. LoxP sites were inserted into near the 5' and 3' ends of the endogenous unc-70 locus to facilitate conditional or cell-specific knockout of the gene. The 5' loxP site can be detected by PCR using the primers 5' tttattaatctatgatttttcagcaaaa 3' and 5' tgacgataatctcttaaaattttgc 3'. The 3' loxP site can be detected by PCR using the primers 5' acgtactgtcgctgaggttacc 3' and 5' gacgtcgatacaaataattcgtccca 3'. Reference: Das R, et al. Sci Adv. 2021 Sep 17;7(38):eabg4617. doi: 10.1126/sciadv.abg4617. PMID: 34533987
MutagenNo mutagen
Made byRavi Das
Laboratory MSB
Reference Ravi Das, Li-Chun Lin, Frederic Català-Castro, Nawaphat Malaiwong, Neus Sanfeliu-Cerdán, Montserrat Porta-de-la-Riva, Aleksandra Pidde, Michael Krieg. An asymmetric mechanical code ciphers curvature-dependent proprioceptor activity. Sci Adv. 2021 Sep; 7(38): eabg4617. doi: 10.1126/sciadv.abg4617
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