Strain Information
| Name | MQD2491 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | daf-16(hq389[daf-16::gfp::degron]) I; ieSi57 II; daf-2(e1370) III. |
| Description | ieSi57 [eft-3p::TIR1::mRuby::unc-54 3' UTR + Cbr-unc-119(+)] II. Maintain at 15C. GFP tag and degron inserted at the 3' end of the endogenous daf-16 gene locus by CRISPR/Cas9 engineering.This strain can be used for auxin-inducible degradation (AID) of DAF-16 protein in somatic tissues. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https://doi.org/10.1101/2021.07.31.454567. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Yan-Ping Zhang, Wen-Hong Zhang |
| Laboratory | MQD |
| Reference | Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https://doi.org/10.1101/2021.07.31.454567. |
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