Strain Information
| Name | MD4574 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(ed3) III; bcSi69 IV; bcEx1370. |
| Description | bcSi69[hsp-16.41p::dCas9::SV40-NLS::HA::GS-linker::SV40-NLS::GFP:: GFP::SV40-NLS::mai-2 3’UTR + Cbr-unc-119(+)] IV. bcEx1370 [U6p::rrn-4(sgRNAs1-4 targeting the rrn-1 locus) + U6p::rrn-4(sgRNAs1-4 targeting the rrn-4 locus) + rol-6(su1006)]. Maintain at 15C. Pick Rollers to maintain. After heat shock, distinct spots of GFP fluorescence are visible in the nucleus. bcEx1370 array contains a PCR product of 4 different guide RNAs targeting rrn-1 (around 100 repeats of rrn-1 on LG I) and a PCR product of 4 different guide RNAs targeting rrn-4 (around 100 repeats of rrn-4 on LG V). Reference: Memar N, et al. In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9. MicroPubl Biol. 2022 Dec 13;2022:10.17912/micropub.biology.000701. doi: 10.17912/micropub.biology.000701. PMID: 36606081; PMCID: PMC9807462. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | Nadin Memar |
| Laboratory | MD |
| Reference | Memar N, Sethi A, Luehr S, Lambie EJ, Conradt B. In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9. MicroPubl Biol. 2022 Dec 13;2022:10.17912/micropub.biology.000701. doi: 10.17912/micropub.biology.000701. PMID: 36606081; PMCID: PMC9807462. |
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