Strain Information
| Name | MD4195 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(ed3) III; bcSi69 IV. |
| Description | bcSi69[hsp-16.41p::dCas9::SV40-NLS::HA::GS-linker::SV40-NLS::GFP:: GFP::SV40-NLS::mai-2 3’UTR + Cbr-unc-119(+)] IV. [NOTE: MD4195 can be maintained at 20C instead of 15C because it does not carry an array with guide RNAs targeting essential genes.] Generated in parental strain EG8081. Reference: Memar N, et al. In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9. MicroPubl Biol. 2022 Dec 13;2022:10.17912/micropub.biology.000701. doi: 10.17912/micropub.biology.000701. PMID: 36606081; PMCID: PMC9807462. |
| Mutagen | MosSCI |
| Outcrossed | x0 |
| Made by | Nadin Memar |
| Laboratory | MD |
| Reference | Memar N, Sethi A, Luehr S, Lambie EJ, Conradt B. In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9. MicroPubl Biol. 2022 Dec 13;2022:10.17912/micropub.biology.000701. doi: 10.17912/micropub.biology.000701. PMID: 36606081; PMCID: PMC9807462. |
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