Strain Information

Name JLFb1   View On Wormbase
Species E. coli
GenotypeE. coli [MG1655 bioB::kan].
DescriptionBiotin auxotrophic E. coli strain is kan resistant and grows fine on LB. This mutant strain produces significantly less biotin and can therefore be used to reduce background in TurboID experiments. Also known as MG1655 bioB::kan and STL110 (J. Cronen, U. of Illinois). References: Sanchez AD & Feldman JL. STAR Protoc. 2021 Dec 2;2(4):100986. doi: 10.1016/j.xpro.2021.100986. PMID: 34927095. Ortega-Cuellar D., et al. J Nutrigenet Nutrigenomics. 2010;3(1):18-30. doi: 10.1159/000318054. PMID: 20798549
Made byOrtega-Cuellar et al., 2010
Laboratory JLF
Reference Sanchez et al., 2021 Ortega-Cuellar et al., 2010
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