Strain Information
| Name | JLFb1 View On Wormbase |
|---|---|
| Species | E. coli |
| Genotype | E. coli [MG1655 bioB::kan]. |
| Description | Biotin auxotrophic E. coli strain is kan resistant and grows fine on LB. This mutant strain produces significantly less biotin and can therefore be used to reduce background in TurboID experiments. Also known as MG1655 bioB::kan and STL110 (J. Cronen, U. of Illinois). References: Sanchez AD & Feldman JL. STAR Protoc. 2021 Dec 2;2(4):100986. doi: 10.1016/j.xpro.2021.100986. PMID: 34927095. Ortega-Cuellar D., et al. J Nutrigenet Nutrigenomics. 2010;3(1):18-30. doi: 10.1159/000318054. PMID: 20798549 |
| Mutagen | N/A |
| Outcrossed | x0 |
| Made by | Ortega-Cuellar et al., 2010 |
| Laboratory | JLF |
| Reference | Sanchez et al., 2021 Ortega-Cuellar et al., 2010 |
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