Strain Information

Name JEL1000   View On Wormbase
Species C. elegans
Genotypehsr-9(xoe17) I.
DescriptionSuperfically wild-type. hsr-9(xoe17) was generated by incorporating a stop-in cassette early in the coding region using the co-CRISPR method (Paix et al. 2015). The hsr-9 repair template(gattttgcctcttaaataaaatttcagCAAAAAACCGAGGGGAGACTTGCAATAGGGAA
GTTTGTCCAGAGCAGAGGTGACTAAGTGATAAGCTAGCTCTCGGATCATCTTGCAAACAT
GCTTATTGCTGgtaggtattgcaacc)
and guide RNA (AGGGGAGACTTGCAATATCT) were injected into N2 and the resulting progeny were analyzed by PCR using TGAAATTAAGGTGGTCACTCGAAG and GTTGTTGTGGGGAGGCTGAA. Reference: Hariri S, et al. (2023). 53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans. microPublication Biology. 10.17912/micropub.biology.000934. PMID: 37581122.
MutagenCrispr/Cas9
Outcrossedx3
Made bySara Hariri
Laboratory JEL
Reference Hariri, S; Li, Q; Engebrecht, J (2023). 53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans. microPublication Biology. 10.17912/micropub.biology.000934. PMC ID: PMC10423319. PubMed ID: 37581122.
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