Strain Information
| Name | JDW979 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | col-61(wrd415[col-61::3xGFP11::3xFLAG]) I; wrdSi142 II. |
| Description | wrdSi142 [loxP eft-3p::ssGFP1-10:::ubl-1 3'UTR FRT3] II. ssGFP1-10 contains an N-terminal signal sequence from OIG-1. Modular linker::3xGFP11::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-61 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Derived by sequential edits of parental strain NM5546: wrdSi133 was an insertion into jsSi1726, and subsequent excision left wrdSi142 [loxP::eft-3p::ssGFP1-10::ubl-1 3'UTR::FRT3] inserted at the former jsSi1726 site. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Jordan Ward |
| Laboratory | JDW |
| Reference | Unpublished, will add citation when strain is preprinted and/or published. |
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