Strain Information
| Name | JDW850 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | col-75(wrd349[col-75::mNG::3xFLAG]) II. |
| Description | Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-75 locus by CRISPR. Allele obtained using plasmid-based unc-119 selection in a temperature-sensitive unc-119(kst33) III background The unc-119(+) cassette was removed through Cre-mediated excision and the unc-119(kst33) allele was removed by outcrossing. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Jordan Ward |
| Laboratory | JDW |
| Reference | Manuscript in preparation. |
Sign in
or
register an account if you want to order this strain.