Strain Information
Name | JDW224 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | wrdSi22 I. |
Description | wrdSi22 [^SEC^eft-3p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). SEC spotaneously excises at a high frequency so non-rollers will appear. Pick Rollers to maintain. Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Self-excising cassette with Rol and HygroR markers still in strain to facilitate crosses. Heat-shock to remove SEC as described in Dickinson et al. 2015. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 |
Mutagen | Crispr/Cas9 |
Outcrossed | x2 |
Made by | Matt Ragle |
Laboratory | JDW |
Reference | Ashley et al., 2020 |
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