Strain Information
| Name | JDW220 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | wrdSi18 I. |
| Description | wrdSi18 [^SEC^mex-5p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Germline and early embryo-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in germline and early embryo nuclei. Self-excising cassette with Rol and HygroR markers still in strain to facilitate crosses. Heat-shock to remove SEC as described in Dickinson et al. 2015. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Matt Ragle |
| Laboratory | JDW |
| Reference | Ashley et al., 2020 |
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