Strain Information
| Name | HCC31 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | hwSi13 II; unc-119(ed9) III. |
| Description | hwSi13 [pie-1p::GFP::mex-3(2-636)::pie-1 3'UTR + unc-119(+)] II. Worms are healthiest at 15C. Shift to 25C overnight to increase brightness of GFP. Mos1-mediated single-copy insertion (MosSCI) into EG4322. GFP-tagged transgene expressing truncated MEX-3 that is missing a portion required for protein degradation. GFP::MEX-3(2-636) is extraordinarily stable, persisting in somatic cells through the comma stage (~550 cells) at levels similar to those in 1- and 2-cell embryos. Also detected on P granules through hatching. Reference: Huang NN & Hunter CP. Gene. 2015 Jan 10;554(2):160-73. |
| Mutagen | MosSCI |
| Outcrossed | x1 |
| Made by | Nancy N. Huang |
| Laboratory | HCC |
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