Strain Information
| Name | HCC27 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | hwSi8 II; unc-119(ed9) III. |
| Description | hwSi8 [pie-1p::GFP::mex-3(601-1248)::pie-1 3'UTR + unc-119(+)] II. Worms are healthiest at 15C. Shift to 25C overnight to increase brightness of GFP. Mos1-mediated single-copy insertion (MosSCI) into EG4322. GFP-tagged transgene expressing a portion of MEX-3(601-1248) required for protein degradation. At about the 12-cell stage, soma-germline asymmetry is briefly visible before GFP::MEX-3(601-1248) becomes undetectable even in the germline blastomeres. Detected weakly on P granules only in the presence of endogenous MEX. Reference: Huang NN & Hunter CP. Gene. 2015 Jan 10;554(2):160-73. |
| Mutagen | MosSCI |
| Outcrossed | x1 |
| Made by | Nancy N. Huang |
| Laboratory | HCC |
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