Strain Information
Name | HCC21 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | hwSi3 II; unc-119(ed9) III. |
Description | hwSi3 [pie-1p::GFP::mex-3::pie-1 3'UTR + unc-119(+)] II. Worms are healthiest at 15C. Shift to 25C overnight to increase brightness of GFP. Mos1-mediated single-copy insertion (MosSCI) into EG4322. GFP-tagged transgene expressing full-length MEX-3 uniformly throughout oocytes and early embryos through the 4-cell stage. GFP::MEX-3 then mirrors endogenous MEX-3, disappearing from somatic cells by about the 12-cell stage. Also detected in P granules. GFP::MEX-3 can replace endogenous MEX-3. Reference: Huang NN & Hunter CP. Gene. 2015 Jan 10;554(2):160-73. |
Mutagen | MosSCI |
Outcrossed | x1 |
Made by | Nancy N. Huang |
Laboratory | HCC |
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