Strain Information

Name GLW95   View On Wormbase
Species C. elegans
GenotypeF13E6.1(utx75[F13E6.1::mNG::3xFlag]) X.
DescriptionC-terminal tag of F13E6.1 via CRISPR/Cas9 knock-in of mNeonGreen at F13E6.1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ AAATCGTGCTCTCCCAAGCA 3’; rev – 5’ CTTGTCACCTGACGGGATGT 3’. Left flank: 5' CCAGTTGCGGAGGAGGCGAAGCCAATCTCT 3' (1 silent mutation); Right flank: 5' TAAattcattcatttcacataccaatatgt 3'; sgRNA: atgaatTTAAGAGATTGGCT; Cas9/sgRNA plasmid: pGLOW26; mNG^SEC^3xFlag plasmid: pGLOW104; SEC insertion allele strain: GLW94.
Made byGonzalo Botello-Lins (Glow Worms '20)
Laboratory GLW
Reference N/A
Sign in or register an account if you want to order this strain.