Strain Information
| Name | GLW93 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | clic-1(utx73[mScarlet-I-C1::3xMyc::clic-1]) V. |
| Description | N-terminal tag of CLIC-1 via CRISPR/Cas9 knock-in of mScarlet at clic-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ CTCGTACCAATCGTCCGCAA 3’; rev – 5’ CCATCTTGTTGCTTGGCGAAA 3’. Left flank: 5' ccagggtataaaacacaaaaaaactacaaa 3'; Right flank: 5' ATGTCGGATCCAGTCGCGGATTTTTTGGCT 3’ (1 silent mutation); sgRNA: ctacaaaATGTCGGATCCAG; Cas9/sgRNA plasmid: pGLOW128; mScarlet^SEC^3xMyc plasmid: pGLOW156; SEC insertion allele strain: GLW92. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Cindy Hu (Glow Worms '22) |
| Laboratory | GLW |
| Reference | N/A |
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