Strain Information

Name GLW91   View On Wormbase
Species C. elegans
Genotypehrpa-1(utx71[hrpa-1::mScarlet-I-C1::3xMyc]) IV.
DescriptionC-terminal tag of HRPA-1 via CRISPR/Cas9 knock-in of mScarlet at hrpa-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ GTCTCCACCAAACGCCTGTA 3’ ; rev – 5’ CTAGCCTGTGTCCCATCAGC 3’. Left flank: 5' AATGGGCCCACGCCCAGGGAGGAAACAGAAACTAT 3' (5 silent mutations); Right flank: 5' TAAattaattccttaagcccctctaagtgt 3’; sgRNA: CAGTGGGCTCATGCTCAAGG; Cas9/sgRNA plasmid: pGLOW144; mScarlet^SEC^3xMyc plasmid: pGLOW153; SEC insertion allele strain: GLW90.
Made byAyla Saeed (Glow Worms '22)
Laboratory GLW
Reference N/A
Sign in or register an account if you want to order this strain.