Strain Information
| Name | GLW91 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | hrpa-1(utx71[hrpa-1::mScarlet-I-C1::3xMyc]) IV. |
| Description | C-terminal tag of HRPA-1 via CRISPR/Cas9 knock-in of mScarlet at hrpa-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ GTCTCCACCAAACGCCTGTA 3’ ; rev – 5’ CTAGCCTGTGTCCCATCAGC 3’. Left flank: 5' AATGGGCCCACGCCCAGGGAGGAAACAGAAACTAT 3' (5 silent mutations); Right flank: 5' TAAattaattccttaagcccctctaagtgt 3’; sgRNA: CAGTGGGCTCATGCTCAAGG; Cas9/sgRNA plasmid: pGLOW144; mScarlet^SEC^3xMyc plasmid: pGLOW153; SEC insertion allele strain: GLW90. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Ayla Saeed (Glow Worms '22) |
| Laboratory | GLW |
| Reference | N/A |
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