Strain Information

Name GLW8   View On Wormbase
Species C. elegans
Genotypeeel-1(utx8[mNG::eel-1]) IV.
DescriptionSlow growing. N-terminal tag of EEL-1 via CRISPR/Cas9 knock-in of mNeonGreen at eel-1 locus. Insertion verified by PCR. Left flank: 5' gacaagcgggttttttgaatcgtttcgaaa 3'; Right flank: 5' ATGAAGATTGACGATGCTGAGCCGAGCTCTAGTTCATCGGGCTCCGATAT 3' (6 silent mutations). sgRNA: 5' GACCCGGAAGAGCTTGAACT 3'
MutagenCRISPR/Cas9 homologous recombination
Made byElla DeMott (Glow Worms '20)
Laboratory GLW
Reference N/A
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