Strain Information
Name | GLW8 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | eel-1(utx8[mNG::eel-1]) IV. |
Description | Slow growing. N-terminal tag of EEL-1 via CRISPR/Cas9 knock-in of mNeonGreen at eel-1 locus. Insertion verified by PCR. Left flank: 5' gacaagcgggttttttgaatcgtttcgaaa 3'; Right flank: 5' ATGAAGATTGACGATGCTGAGCCGAGCTCTAGTTCATCGGGCTCCGATAT 3' (6 silent mutations). sgRNA: 5' GACCCGGAAGAGCTTGAACT 3' |
Mutagen | CRISPR/Cas9 homologous recombination |
Outcrossed | x0 |
Made by | Ella DeMott (Glow Worms '20) |
Laboratory | GLW |
Reference | N/A |
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