Strain Information

Name GLW79   View On Wormbase
Species C. elegans
Genotypesod-5(utx61[mScarlet-I-C1::3xMyc::sod-5]) II.
DescriptionN-terminal tag of SOD-5 via CRISPR/Cas9 knock-in of mScarlet at sod-5 locus. Insertion verified by PCR and fluorescence. Left flank: 5' tgtgctaacgaaaattttactaaaaggaaa 3'; Right flank: 5' ATGGATATTCTCTCTGATATTGCAAATGCC 3’ (1 silent mutation); sgRNA: tacCTGTGGAAGAACGGCAT; Cas9/sgRNA plasmid: pGLOW122; mScarlet^SEC^3xMyc plasmid: pGLOW129; SEC insertion allele strain: GLW78.
Made byGaby Sobrevilla (Glow Worms '22)
Laboratory GLW
Reference N/A
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