Strain Information

Name GLW73   View On Wormbase
Species C. elegans
GenotypeH34C03.2(utx55[mNG::3xFlag::H34C03.2]) IV.
DescriptionN-terminal tag of H34C03.2 via CRISPR/Cas9 knock-in of mNeonGreen at H34C03.2 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gggattgcctacactcaaatatacgtaacg 3'; Right flank: 5' ATGCCCACGCCGGAAGTGTTCCCATGGATG 3’ (4 silent mutations); gRNA: cgtaacgATGCCAACTCCCG; Cas9/sgRNA plasmid: pGLOW9; mNG^SEC^3xFlag plasmid: pGLOW106; SEC insertion allele strain: GLW72.
Made byChristine Collins, Vinay Pillai, Wesley Yeung
Laboratory GLW
Reference N/A
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