Strain Information

Name GLW65   View On Wormbase
Species C. elegans
Genotypesod-2(utx53[mScarlet::3xMyc::sod-2]) I.
DescriptionN-terminal tag of SOD-2 via CRISPR/Cas9 knock-in of mScarlet at sod-2 locus. Insertion verified by PCR and fluorescence. Left flank: 5' attgaatattttaattatttgcagccgaaa 3'; Right flank: 5' ATGCTTCAAAACACGGTTCGCTGTGTCTCA 3’ (1 silent mutation); sgRNA: AAGCTTTGAGACACAGCGAA; Cas9/sgRNA plasmid: pGLOW98; mScarlet^SEC^3xMyc plasmid: pGLOW111; SEC insertion allele strain: GLW64.
MutagenCRISPR/Cas9 homologous recombination
Made byParam Kapoor (Glow Worms '21)
Laboratory GLW
Reference N/A
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