Strain Information

Name GLW63   View On Wormbase
Species C. elegans
Genotypepqn-59(utx51[mNG::3xFlag::pqn-59]) I.
DescriptionN-terminal tag of PQN-59 via CRISPR/Cas9 knock-in of mNeonGreen at pqn-59 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gaccctttttatttataatttgttttcaga 3'; Right flank: 5' ATGGGTATTAAAGGCGACAAAAAAGCTACT 3’ (1 silent mutation); sgRNA: TGCAAGTCGCGCTTGATCGC; Cas9/sgRNA plasmid: pGLOW23; mNG^SEC^3xFlag plasmid: pGLOW24; SEC insertion allele strain (balanced): GLW62.
MutagenCRISPR/Cas9 homologous recombination
Made byPersephone Alicea (Glow Worms '20)
Laboratory GLW
Reference N/A
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