Strain Information

Name GLW6   View On Wormbase
Species C. elegans
GenotypeW08E12.7(utx6[mNG::W08E12.7]) IV.
DescriptionSuperficially wild-type. N-terminal tag of W08E12.7 via CRISPR/Cas9 knock-in of mNeonGreen at W08E12.7 locus. Insertion verified by PCR. Left flank: 5' ttcaatgtttattctttcagatagatcaaa 3'; Right flank: 5' ATGGTTAGAAAGGACAGTGAGAGTAGTTGTTCAAGTGATGG 3' (6 silent mutations). sgRNA: 5' GAAAGCAGCTGCTCCAGCGA 3'
MutagenCRISPR/Cas9 homologous recombination
Made byAubrie Rettmann (Glow Worms '20)
Laboratory GLW
Reference N/A
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