Strain Information
Name | GLW6 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | W08E12.7(utx6[mNG::W08E12.7]) IV. |
Description | Superficially wild-type. N-terminal tag of W08E12.7 via CRISPR/Cas9 knock-in of mNeonGreen at W08E12.7 locus. Insertion verified by PCR. Left flank: 5' ttcaatgtttattctttcagatagatcaaa 3'; Right flank: 5' ATGGTTAGAAAGGACAGTGAGAGTAGTTGTTCAAGTGATGG 3' (6 silent mutations). sgRNA: 5' GAAAGCAGCTGCTCCAGCGA 3' |
Mutagen | CRISPR/Cas9 homologous recombination |
Outcrossed | x0 |
Made by | Aubrie Rettmann (Glow Worms '20) |
Laboratory | GLW |
Reference | N/A |
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