Strain Information
Name | GLW57 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | asp-3(utx47[mNG::3xFlag::asp-3]) X. |
Description | N-terminal tag of ASP-3 via CRISPR/Cas9 knock-in of mNeonGreen at asp-3 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gcgctgcttctcaattagtgataacgcacc 3'; Right flank: 5' ATGTCGGGCCGCGTTTTCCTTCTTCTGGCT 3'; sgRNA: tagtgataacgcaccATGT (19 bp); Cas9/sgRNA plasmid: pGLOW74; mNG^SEC^3xFlag plasmid: pGLOW96; SEC insertion allele strain: GLW58. |
Mutagen | CRISPR/Cas9 homologous recombination |
Outcrossed | x0 |
Made by | Stephen Pullman (Glow Worms '21) |
Laboratory | GLW |
Reference | N/A |
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