Strain Information

Name GLW53   View On Wormbase
Species C. elegans
Genotypeegl-19(utx45[egl-19(A&B)::mScarlet-I-C1::3xMyc] IV.
DescriptionInternal tag of EGL-19 at N-terminal side of exon 3 via CRISPR/Cas9 knock-in of mScarlet at egl-19 locus. Tags isoforms a and b. Insertion verified by PCR and fluorescence. Left flank: 5' ttatttgaatgagcaaaaaataaatttcag 3'; Right flank: 5' GCCGCAGTGGCAGCTTCATCATCACAAGAT 3’ (1 silent mutation); gRNA: TTGTGATGATGAAGCTGCCA; Cas9/sgRNA plasmid: pGLOW69; mScarlet^SEC^3xMyc plasmid: pGLOW60; SEC insertion allele strain (balanced): GLW52.
Made byKara McDonald (Glow Worms '21)
Laboratory GLW
Reference N/A
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