Strain Information

Name GLW51   View On Wormbase
Species C. elegans
Genotypecey-1(utx43[mNG::3xFlag::cey-1]) II.
DescriptionN-terminal tag of CEY-1 via CRISPR/Cas9 knock-in of mNeonGreen at cey-1 locus. Insertion verified by PCR and fluorescence. Left flank: 5' accagaggaagaacagaagcgcgcggaaca 3'; Right flank: 5' ATGGCGGAGAAAAACgtaagtgtgtgtctc 3' (1 silent mutation); sgRNA: acacacacttacGTTTTTCT; Cas9/sgRNA plasmid: pGLOW71; mNG^SEC^3xFlag plasmid: pGLOW93; SEC insertion allele strain (balanced): GLW50.
Made byLiam Schuck (Glow Worms '21)
Laboratory GLW
Reference N/A
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