Strain Information
Name | GLW51 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | cey-1(utx43[mNG::3xFlag::cey-1]) II. |
Description | N-terminal tag of CEY-1 via CRISPR/Cas9 knock-in of mNeonGreen at cey-1 locus. Insertion verified by PCR and fluorescence. Left flank: 5' accagaggaagaacagaagcgcgcggaaca 3'; Right flank: 5' ATGGCGGAGAAAAACgtaagtgtgtgtctc 3' (1 silent mutation); sgRNA: acacacacttacGTTTTTCT; Cas9/sgRNA plasmid: pGLOW71; mNG^SEC^3xFlag plasmid: pGLOW93; SEC insertion allele strain (balanced): GLW50. |
Mutagen | Crispr/Cas9 |
Outcrossed | x0 |
Made by | Liam Schuck (Glow Worms '21) |
Laboratory | GLW |
Reference | N/A |
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