Strain Information

Name GLW45   View On Wormbase
Species C. elegans
GenotypeZK1058.9(utx37[ZK1058.9::mNG::3xFlag]) III
DescriptionC-terminal tag of ZK1058.9 via CRISPR/Cas9 knock-in of mNeonGreen at ZK1058.9 locus. Insertion verified by PCR and fluorescence. Left flank: 5' AGCTCAACGGCTAGCTGGTCTCGTTATTAT 3' (1 silent mutation); Right flank: 5' TAAtgaattttcctccaacttttgtcctct 3'; sgRNA: AATAACGAGACCAGCTAG (18 bp); Cas9/sgRNA plasmid: pGLOW58; mNG^SEC^3xFlag plasmid: pGLOW68; SEC insertion allele strain: GLW44.
MutagenCRISPR/Cas9 homologous recombination
Made byDan Tatulescu (Glow Worms '21)
Laboratory GLW
Reference N/A
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