Strain Information

Name GLW41   View On Wormbase
Species C. elegans
Genotypenhr-8(utx33[mNG::3xFlag::nhr-8]) IV.
DescriptionN-terminal tag of NHR-8 via CRISPR/Cas9 knock-in of mNeonGreen at nhr-8 locus. Insertion verified by PCR and Sanger sequencing. Left flank: 5' taatcactaaaacaaaaatttcgtcattcc 3'; Right flank: 5' ATGCCTTCGTCTTCTCCATCGATGGACGAG 3'; sgRNA: CGATGGAGAAGACGAAGGCA; Cas9/sgRNA plasmid: pGLOW55; mNG^SEC^3xFlag plasmid: pGLOW56; SEC insertion allele strain: GLW40.
MutagenCRISPR/Cas9 homologous recombination
Made byGillian Witten (Glow Worms '21)
Laboratory GLW
Reference N/A
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