Strain Information

Name GLW4   View On Wormbase
Species C. elegans
Genotypegyf-1(utx4[gyf-1::mNG]) II.
DescriptionSuperficially wild-type. C-terminal tag of GYF-1 via CRISPR/Cas9 knock-in of mNeonGreen at gyf-1 locus. Insertion verified by PCR. Left flank: 5' CCATCGGCTCCGGTGAATCCTTCGCGCCGT 3'; Right flank: 5' TAGatgagtcatttctttttccagctttaa 3'. sgRNA: 5' TGACTCATCTAACGGCGCGA 3'
MutagenCRISPR/Cas9 homologous recombination
Made bySara Blanco (Glow Worms '20)
Laboratory GLW
Reference N/A
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