Strain Information

Name GLW39   View On Wormbase
Species C. elegans
Genotypeccdc-47(utx31[mNG::3xFlag::ccdc-47]) III.
DescriptionN-terminal tag of CCDC-47 via CRISPR/Cas9 knock-in of mNeonGreen at ccdc-47 locus. Insertion verified by PCR, Sanger sequencing, and fluorescence. Left flank: 5' gttaaatcactcaatttcgggtcgttcacc 3'; Right flank: 5' ATGAAGATAGTATGGATTTTCCTAATATTC 3' (3 silent mutations); sgRNA: cgttcaccATGAAAATCGTC; Cas9/sgRNA plasmid: pGLOW13; mNG^SEC^3xFlag plasmid: pGLOW17; SEC insertion allele strain (balanced): GLW38.
MutagenCRISPR/Cas9 homologous recombination
Made byAnita Rhodes (Glow Worms '20)
Laboratory GLW
Reference N/A
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